Genetics of cycloheximide resistance in Tetrahymena.
نویسندگان
چکیده
Cycloheximide resistance in Tetrahymena is inducible by mutagenesis. Two mutations, isolated independently from genetically different strains, were analyzed. The two mutations, Chx-1 and Chx-2, were found to be alleles at the same locus. A specific analysis is presented which may serve as a model for strategies employing an independent manipulation of the micro-and macronuclei of this organism for genetic studies.
منابع مشابه
Isolation and genetic characterization of a mutation affecting ribosomal resistance to cycloheximide in Tetrahymena.
A dominant mutation at a new locus affecting resistance to cycloheximide has been isolated by exploiting a synergistic relationship with a previously known mutation for cycloheximide resistance in Tetrahymena. The new mutation (ChxB) was induced in a line homozygous for ChxA and was recovered from that background by a new technique termed interrupted genomic exclusion. Segregation data from the...
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Two cycloheximide-resistant mutants of Tetrahymena thermophila were analyzed to determine the site of their cycloheximide resistance. The mutations in both strains had been previously shown to be genetically dominant and located at separate loci (denoted Chx-A and Chx-B). Strains carrying these mutations were readily distinguished by the extent to which they were resistant to the drug. The homo...
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ورودعنوان ژورنال:
- Genetics
دوره 87 2 شماره
صفحات -
تاریخ انتشار 1977